Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery

Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery

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The aim of the present study was iphone 13 pro max price winnipeg to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection.The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field.Subsequently, the MNPs were evaluated in vivo in young rats.

The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles.The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent.The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen.

In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry il barone wine genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro.